Mass Spectrometry-Based High-Throughput
Proteome
Mapping Core
 University of
Michigan
Michigan State University
Analysis
of protein expression patterns (including quantitation),
identification of post-translational modifications
and analysis of protein complexes.
Protein Microarray Core
Van
Andel Research Institute
Rapid screening of protein expression
patterns and protein interactions.
Yeast 2-Hybrid Core
Wayne State University
Analysis of binary
interactions among large sets of proteins.
Proteome Informatics Core
University of Michigan
PRIME
Development
of unified support of all the proteome technologies
- data processing and analysis, archiving, integration,
and web-based data distribution.
Each of these investigators has strong expertise in these fields
and all are leaders in the development of new technologies.
The technologies comprising the components of the Michigan Proteome Consortium
are all newly developed and rapidly evolving. It is
difficult for any one laboratory to develop and maintain expertise in these areas,
making the development of state-wide core services
imperative if these technologies are to be broadly available to Michigan researchers.
University of Michigan
Mass Spectrometry-Based High-Throughput
Proteome Mapping
Core
Protein maps, quantitative analysis of protein
expression patterns, identification of post-translational
modifications, and analysis of protein complexes.
Proteome Informatics
Development
of unified support for all Consortium technologies—Data
processing and analysis, archiving, integration, and web-based
data distribution.
Michigan State University
Mass Spectrometry-Based High-Throughput Proteome Mapping
Core
Protein maps, quantitative analysis of protein
expression patterns, identification of post-translational
modifications, and analysis of protein complexes.
Van
Andel Research Institute
Proteome Microarrays
This group's role is to perform antibody and protein
microarray analysis to study protein expression profiles
and protein/protein interactions. They use robotically
spotted microarrays of antibodies and proteins on hydrogel-coated
microscope slides. The microarrays are incubated with
fluorescently-labeled protein solutions, and proteins
bind according to specific binding reactions with the
spotted capture reagents. Two differentially labeled
protein solutions are incubated together on the array
to allow a direct comparison of protein abundances.
The microarrays are scanned with a two-color microarray
scanner.
The lab is equipped with a robotic microarrayer, a
two-color laser scanner, a Beckman Biomek FX liquid
handling robot, and high-throughput PCR machines.
Wayne State University
Yeast 2-Hybrid Core
Analysis of binary
interactions among large sets of proteins.
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University of Michigan
Michigan State University
Van Andel Research Institute
Wayne State University |
