References and Suggested Readings
Journals
Appel RD, Sanchez JC, Bairoch A, Golaz O, Ravier F, Pasquali C, Hughes GJ, Hochstrasser DF. The SWISS-2DPAGE database of two-dimensional polyacrylamide gel electrophoresis, its status in 1995. Nucleic Acids Res. 1996 Jan 1;24(1):180-1
Appel RD, Sanchez JC, Bairoch A, Golaz O, Ravier F, Pasquali C, Hughes GJ, Hochstrasser DF. The SWISS-2DPAGE database of two-dimensional polyacrylamide gel electrophoresis. Nucleic Acids Res. 1994 Sep;22(17):3581-2.
Bjellqvist B, Hughes GJ, Pasquali C, Paquet N, Ravier F, Sanchez JC, Frutiger S, Hochstrasser D. The focusing positions of polypeptides in immobilized pH gradients can be predicted from their amino acid sequences. Electrophoresis. 1993 Oct;14(10):1023-31.
Bjellqvist B, Pasquali C, Ravier F, Sanchez JC, Hochstrasser D. A nonlinear wide-range immobilized pH gradient for two-dimensional electrophoresis and its definition in a relevant pH scale. Electrophoresis. 1993 Dec;14(12):1357-65.
Bjellqvist B, Sanchez JC, Pasquali C, Ravier F, Paquet N, Frutiger S, Hughes GJ, Hochstrasser D. Micropreparative two-dimensional electrophoresis allowing the separation of samples containing milligram amounts of proteins. Electrophoresis. 1993 Dec;14(12):1375-8
Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248-54.
Gorg A, Obermaier C, Boguth G, Harder A, Scheibe B, Wildgruber R, Weiss W. The current state of two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis. 2000 Apr;21(6):1037-53.
Hochstrasser DF, Frutiger S, Paquet N, Bairoch A, Ravier F, Pasquali C, Sanchez JC, Tissot JD, Bjellqvist B, Vargas R, et al. Human liver protein map: a reference database established by microsequencing and gel comparison. Electrophoresis. 1992 Dec;13(12):992-1001.
Mortz E, Krogh TN, Vorum H, Gorg A. Improved silver staining protocols for high sensitivity protein identification using matrix-assisted laser desorption/ionization-time of flight analysis. Proteomics. 2001 Nov;1(11):1359-63.
Rabilloud T. Solubilization of proteins for electrophoretic analyses. Electrophoresis. 1996 May;17(5):813-29.
Rabilloud T, Adessi C, Giraudel A, Lunardi J. Improvement of the solubilization of proteins in two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis. 1997 Mar-Apr;18(3-4):307-16.
Rabilloud T, Strub JM, Luche S, van Dorsselaer A, Lunardi J. A comparison between Sypro Ruby and ruthenium II tris (bathophenanthroline disulfonate) as fluorescent stain for protein detection in gels. Proteomics. 2001 May;1(5):699-704.
Rabilloud T, Vuillard L, Gilly C, Lawrence JJ. Silver-staining of proteins in polyacrylamide gels: a general overview. Cell Mol Biol. 1994 Feb;40(1):57-75.
Books
2-D Proteome Analysis Protocols in Methods in Molecular Biology. (1999) Link, AJ, ed. Humana Press, Totowa, New Jersey.
Berkelman, T and Stenstedt, T (1998) 2-D electrophoresis using immobilized pH gradients: Principles and Methods. Amersham Bioscience, Piscataway, New Jersey.
Current Protocols in Protein Science. (2003) Coligan JE, et al., Eds. John Wiley & Sons, Inc, New York
Dunbar BS. (1987) Two dimensional electrophoresis and immunological techniques. Plenum Press, New York.
Hanash SM, Strahler JR. (1991) Immobilized pH gradients: analytical and preparative use. Companion to Methods in Enzymology, 3, 109-114.
Harrington MG, Gudeman T, Zewert T, Yun M, Hood L. (1991) Analytical and micropreparative two-dimensional electrophoresis of proteins. Companion to Methods in Enzymology, 3, 98-108.
Protein-Protein Interactions: A Molecular Cloning Manual (2002) Golemis E, Ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
Righetti PG. (1990) Immobilized pH gradients: theory and methodology in Laboratory techniques in biochemistry and molecular biology. Elsevier Press, Amsterdam.
Wilkins MR, Williams RD, Hochstrasser RD. (1997) Proteome research: new frontiers I functional genomics. Springer, Berlin.
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Protocols
The Michigan Proteome Consortium follows a standard set of protocols listed below:
Whole cell lysate
Membrane proteins
IPG 1st dimension
SDS page
Colloidal coomassie stain
Fluorescence stain
Silver stain
In-gel digestion
References and suggested reading
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